MALDI-ToF Analysis of Mitochondrial Peptides

Abstract

Abstract: Short peptides are known to play an important role in modulating transcription, in transmitting biological information and in restoring the genetic alterations that occur with aging. This paper aims to describe a method of identifying the population of peptides within a peptide cocktail formulation. A sample of lung-derived Mito Organelle (MO) Peptides (LBS) of specific pathogen free (SPF) mammalian rabbits sourced from Charles River Labs were analyzed by mass spectrometry and chromatograms were generated for further examination. The experimentally derived peaks were compared between two batches of LBS MO peptides using MALDI-ToF mass spectroscopy. The following report outlines the experimental methods and the results from performing MALDI-ToF mass spectrometry on various peptides from the company European Wellness (EW).